Figure 5.
Superoxide accumulation in C. glabrata BG2 biofilm cells upon treatment with MCZ-DB and corresponding fungicidal activity. C. glabrata BG2 biofilms were treated with 500 μM MCZ, 37.5 μM DB or a combination of both. DMSO background concentration was 1%. Fluorescence at λex 510 nm and λem 595 nm was normalized for the number of CFU/biofilm. Mean values ± SEM are shown for seven biological repeats. Statistical analysis was performed to assess whether there is a significant increase in fluorescence units/1,000 CFU and a significant decrease in the number of CFU/biofilm upon treatment with MCZ-DB as compared to DMSO control treatment and both single compound treatments. A two-way ANOVA and Dunnett's or Tukey's multiple comparison test was applied and significant differences (p < 0.01) compared to single compound treatments and the DMSO control treatment are shown in orange. **p < 0.01; ****p < 0.0001.