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. 2021 Jan 21;9:617225. doi: 10.3389/fcell.2021.617225

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Copyright © 2021 Ge, Li, Sun, Xin, Zhu, Liu, Tang, Han, Huang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell apoptosis of blastocysts derived from HFD oocytes. (A) TUNEL analysis of blastocysts originated from ND and HFD oocytes. Embryos were labeled with Hoechst 33342 (blue) for DNA and by TUNEL for fragmented DNA (red). Scale bars, 25 μm. (B) Quantification of ND and HFD blastocysts apoptosis with TUNEL positive nuclei (n = 20 embryos from 5 mice for each group). Data are expressed as mean percentage ± SD, a Student’s t-test was used for statistical analysis. (C) Diagram summarizing the main effects of maternal obesity on telomeric status and genomic stability in oocytes and early embryos.