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Schematic model of the autophagy process. The process of autophagy includes four steps: (i) initiation/nucleation where the phagophore is formed and starts engulfing the cargo, (ii) formation of the phagosome by elongation and maturation of the phagophore, (iii) fusion of the phagosome with the lysosome, and (iv) degradation of the internal material by lysosomal hydrolases. Bafilomycin A1 is used to measure autophagy flux through the inhibition of autophagolysosome formation.
