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. 2021 Jan 21;8:620409. doi: 10.3389/fcell.2020.620409

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Copyright © 2021 Campanario, Ramírez-Pardo, Hong, Isern and Muñoz-Cánoves.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Satellite cell isolation by FACS and subsequent analysis of autophagy through flow cytometry. (A) Representative example of the FACS strategy and gating scheme to isolate quiescent SCs (QSCs) rom resting muscles. (B) Representative example of histogram of LC3-GFP intensity (left panel) and analysis of the mean fluorescence intensity (MFI) by flow cytometry (right panel) in QSCs treated for 4 h with vehicle (DMSO) or BafA1 prior to their isolation by FACS (n = 4). Mean ± SD; two-tailed unpaired t-test. *p < 0.05.