Fig. 9.

SOX10 was a direct target for miR‐637 in glioma cells. (A–D) The expression levels of SOX10 were measured by qRT‐PCR (A,B) or by Western blotting (C,D) in LN229 or SHG‐44 cells treated with miR‐637 mimics or inhibitor. (E) Bioinformatic analysis identified a potential binding site between miR‐637 and the 3’‐UTR sequences of SOX10. (F) The luciferase activity was measured by reporter assay. Wild‐type (WT) or mutated (MUT) SOX10 binding sequence was cloned into the reporter construct, co‐transfected into LN229 or SHG‐44 cells with either miR‐637 mimics or inhibitor. Data are presented as mean ± SD from three independent experiments. Comparison between two groups was performed using Student’s t‐test and between three or more groups using one‐way ANOVA followed by Tukey’s post hoc test. *P < 0.05, **P < 0.01.