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. 2020 Dec 1;15(2):503–522. doi: 10.1002/1878-0261.12845

Fig. 6.

Fig. 6

Expression and activation of the EGF‐R in EOC αvβ3‐TMD transfectants grown suspended in ascites. The expression of total EGF‐R (A) and activated EGF‐R (p‐EGF‐R) (B) was detected by western blot analysis after cell suspension for 3, 24, and 48 h, respectively. Depicted are typical western blots together with corresponding histograms each summarizing the mean values of GAPDH‐normalized fluorescence signal intensities ± SD (n = 3) by setting the values obtained for TMD‐αvβ3 transfectants at each time point to ‘1’. Significance was tested by two‐way ANOVA with post hoc Bonferroni t‐test. Significance is indicated by *P < 0.050, **P < 0.010, and ***P < 0.001.