Fig. 7.

Expression and activation of integrin‐related signaling molecules in EOC αvβ3‐TMD transfectants. The expression levels of FAK (A), p‐FAK (B), PKB/Akt (E), and p‐PKB/Akt (F), respectively, were detected in cells grown suspended or adherent (ad) to cell culture plates for 48 h, by western blot analysis. Typical western blots are depicted together with corresponding histograms summarizing the mean values of GAPDH‐normalized fluorescence signal intensities ± SD from a minimum of 3 independent experiments as n‐fold, by setting the values obtained for TMD‐αvβ3 expressers under each culture condition to ‘1’. For the expression of src (C) and p‐src (D) representative western blots are shown together with histograms outlining the corresponding relative signal intensities by setting that in TMD‐αvβ3 to ‘1’. The kinases p44/p42^erk1/2 (G), and p‐p44/p42^erk1/2 (H), respectively, were determined by western blot analysis in cell transfectants cultured for 48 h suspended in ascites as described under (A). Representative western blots are depicted together with the corresponding histograms from 3 independent experiments as described under (A). Significance was tested by two‐way ANOVA with post hoc Bonferroni t‐test. Significance is indicated by *P < 0.050, **P < 0.010, and ***P < 0.001.