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. 2021 Feb 3;11:2926. doi: 10.1038/s41598-021-82378-y

Table 2.

Group comparison between patients with and without bacterial bile duct colonization.

Bacterial colonization (n = 80) No bacterial colonization (n = 15) p value
Gender, m/f (%) 58 (72.5)/22 (27.5) 13 (86.7)/2 (13.3) .247
Age (years) 69 (56–73) 67 (55–73) .846
BMI (kg/m2) 25 (22–29) 25 (23–27) .988
Portal vein embolization, n (%) 35 (43.8) 8 (53.3) .494
ASA, n (%) .426
 I 5 (6.3) 0
 II 28 (35.0) 8 (53.3)
 III 44 (55.0) 7 (46.7)
 IV 3 (3.8) 0
 V 0 0
Bismuth type, n (%) .095
 I 2 (2.5) 2 (13.3)
 II 8 (10.0) 2 (13.3)
 IIIa 22 (27.5) 7 (46.7)
 IIIb 17 (21.3) 2 (13.3)
 IV 31 (38.8) 2 (13.3)
EBD, n (%) 73 (91.3) 9 (60.0) .001
PBD, n (%) 23 (28.8) 3 (20.0) .485
Preoperative cholangitis, n (%) 30 (37.5) 2 (13.3) .069
Preoperative antibiotics, n (%) 36 (45.0) 6 (40.0) .720
Antibiotic type, n (%) .990
 Ciprofloxacin 23 (63.9) 5 (83.3)
 Piperacillin/tazobactam 5 (13.9) 1 (16.7)
 Others 8 (22.2) 0
Bacterial bile duct colonization, n (%) 80 (100) 0
Most common bacteria, n (%) n. a
 Enterococcus faecalis 31 (38.8)
 Enterococcus faecium 26 (32.5)
 Enterobacter cloacae 13 (16.3)
 Escherichia coli 9 (11.3)
 Klebsiella pneumoniae 6 (7.5)
 Staphylococcus epidermidis 6 (7.5)
 Klebsiella oxytoca 5 (6.3)
 Hafnia alvei 4 (5.0)
 Citrobacter freundii 4 (5.0)
Susceptibility to preoperative antibiotics, n (%) 8 (25.0) n. a
Susceptibility to applied perioperative antibiotics, n (%) 10 (12.5) n. a
Postoperative abdominal infections, n (%) 45 (56.3) 7 (46.7) .494
Other postoperative infections, n (%) 17 (21.3) 1 (14.3) .186
Abdominal infections bacteria, n (%)
 Enterococcus faecium 32 (71.1) 5 (71.4) .986
 Enterococcus faecalis 12 (26.7) 4 (57.1) .104
 Enterobacter cloacae 12 (26.7) 1 (14.3) .482
 Escherichia coli 9 (20.0) 1 (14.3) .721
 Klebsiella pneumoniae 7 (15.6) 2 (28.6) .397
 Staphylococcus epidermidis 4 (8.9) 1 (14.3) .652
 Klebsiella oxytoca 3 (6.7) 0 .482
 Serratia marcescens 1 (2.2) 2 (28.6) .005
Intensive care, days 2 (1–9) 2 (1–4) .715
Hospitalization, days 22 (13–41) 18 (21–31) .345
Postoperative complications, n (%) .813
 Clavien–Dindo ≤ IIIa 40 (50.0) 7 (46.7)
 Clavien–Dindo ≥ IIIb 40 (50.0) 8 (53.3)
30-day mortality, n (%) 13 (16.3) 1 (6.7) .337

The various agents described here as “Preoperative antibiotics” describe the preoperative administration and do not refer to perioperative antimicrobial prophylaxis. Data presented as median and interquartile range if not noted otherwise. Bold indicates statistical significance (p < 0.05).

ASA American society of anesthesiologists classification, BMI body mass index, EBD endoscopic biliary drainage, PBD percutaneous biliary drainage, PVE portal vein embolization.