Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 3;11:2854. doi: 10.1038/s41598-021-82499-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Localization of PfM1AAP and PfM17LAP in intra-erythrocytic P. falciparum 3D7 trophozoite-stage parasites. Immunofluorescence assays were carried out using air-dried blood smears fixed with 75% acetone and 25% methanol at − 20 °C for 5 min, or 50% ethanol and 50% methanol at − 20 °C for 2 min, or 4% PFA and 0.0075% glutaraldehyde for 20 min at room temperature. Fixed parasites were probed with polyclonal antibodies against (A) PfM1AAP and (B) PfM17LAP. Specific aminopeptidase staining (green, Alexa-Fluor 488) was observed in the cytosol of parasites. Parasite nuclei were visualized using DAPI (blue; 4,6-diamidino-2-phenylindole) and monoclonal antibodies against the DV marker plasmepsin I (α-plasmepsin I, red, Alexa-Fluor 594) were used as a control. Differential interference contrast (DIC) and α-plasmepsin I with DIC are shown for reference. Scale bar, 3 µm.