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. 2020 Oct 14;11(3):841–855. doi: 10.1016/j.jcmgh.2020.10.001

Figure 3.

Figure 3

Acid-induced release of PEA and PPARα expression in duodenal mucosa. (A) Representative chromatography coupled to tandem mass spectrometry analysis and (B) relative quantification of PEA levels (expressed as nanomolar concentration in duodenal homogenates) from mucosa of 20 dyspeptic and 10 control biopsy specimens cultured at pH 3.0 and 7.4, respectively, and in the presence or absence of TTX. (C) Immunoblot analysis and relative densitometric analysis (arbitrary units normalized on the expression of the housekeeping protein β-actin) showing PPARα protein expression in tissue homogenates from 10 and 6 dyspeptic and control subjects, respectively. ∗∗∗P < .001 vs control; °P < .05 FD untreated vs pretreatment with TTX; ###P < .05 control untreated vs pretreatment with TTX. All results are expressed as means ± SD.