Table 1:
The top-10 potential upstream regulators identified through IPA Upstream Regulator Analysis
| Top-10 regulators | Description | Number of target genes in dataset | Predicted activation state | Activation z-score | P-value of overlap |
|---|---|---|---|---|---|
| E-Wnt cells | |||||
| lipopolysaccharide | microbial component | 27 | Inhibited | −3.11 | 2.55E-10 |
| bicuculline | chemical - endogenous | 7 | Inhibited | −2.57 | 1.09E-09 |
| dalfampridine | chemical drug | 7 | Inhibited | −2.65 | 1.81E-09 |
| GnRH-A | chemical reagent | 7 | Inhibited | −2.61 | 7.54E-09 |
| Fosll | transcription regulator | 7 | Activated | 2.18 | 1.01E-08 |
| Igf1 | growth factor | 13 | Inhibited | −3.07 | 1.72E-08 |
| Ifng | cytokine | 21 | Inhibited | −2.01 | 1.75E-08 |
| Pdgfbb | complex | 11 | Inhibited | −3.24 | 2.88E-08 |
| beta-estradiol | chemical - endogenous | 24 | Inhibited | −2.63 | 7.08E-08 |
| Creb1 | transcription regulator | 13 | Inhibited | −2.90 | 1.24E-07 |
| M-Wnt cells | |||||
| lipopolysaccharide | microbial component | 192 | Activated | 6.62 | 1.23E-13 |
| Mapk1 | kinase | 60 | Inhibited | −2.28 | 1.60E-13 |
| Irf7 | transcription regulator | 39 | Activated | 5.83 | 2.27E-13 |
| Ifna2 | cytokine | 46 | Activated | 6.12 | 1.57E-12 |
| Ifnl1 | cytokine | 26 | Activated | 5.01 | 1.73E-12 |
| Tp53* | transcription regulator | 187 | Activated | 4.35 | 1.85E-12 |
| Pntp1 | enzyme | 20 | Inhibited | −4.35 | 2.20E-12 |
| Trim24 | transcription regulator | 28 | Inhibited | −4.29 | 2.74E-12 |
| Irf3 | transcription regulator | 41 | Activated | 5.34 | 2.76E-12 |
| Interferon alpha | group | 66 | Activated | 5.51 | 6.18E-12 |
| metM-Wntllver cells | |||||
| Stat3 | transcription regulator | 25 | Activated | 3.00 | 2.85E-12 |
| Prl | cytokine | 18 | Inhibited | −2.52 | 5.52E-12 |
| Irf7 | transcription regulator | 14 | Inhibited | −3.66 | 4.94E-11 |
| Ifnb1 | cytokine | 16 | Inhibited | −2.77 | 8.31E-10 |
| dexamethasone | chemical drug | 43 | Activated | 2.11 | 8.55E-10 |
| Ifnl1 | cytokine | 10 | Inhibited | −3.10 | 8.87E-10 |
| Ackr2 | G-protein coupled receptor | 8 | Activated | 2.83 | 1.04E-09 |
| Mapk1 | kinase | 17 | Activated | 3.80 | 1.71E-09 |
| Stat6 | transcription regulator | 18 | Activated | 3.13 | 3.59E-09 |
| Ifna2 | cytokine | 14 | Inhibited | −3.18 | 4.50E-09 |
The top-10 potential upstream regulators based on P-value of overlap in regulators with a z-score of <−2 or >2 were considered. Predicted regulators that were flagged for potential bias were excluded.
*
Trp53 is the mouse variant of the common (human) TP53 output obtained through Ingenuity Pathway Analysis.