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. 2021 Feb 3;11:2979. doi: 10.1038/s41598-021-82272-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Principal coordinates analysis (PCoA) based on Bray–Curtis similarity of pyrosequencing data. F4–F14: samples from the freshwater reactor (FR); S3–S13: samples from seawater reactor (SR). (A) Based on total OTUs; (B) Based only on OTUs representing the AOB guild; (C) Based on OTUs representing the NOB guild, and (D) Based on OTUs representing the nitrifying community (AOB plus NOB guilds). Four groups of samples are identified FR_start, FR_end, SR_start, and SR_end. The arrows between each group show the time line for sampling.