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. 2021 Feb 3;11:2979. doi: 10.1038/s41598-021-82272-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Diversity indices based on the pyrosequencing data of the total and the nitrifying communities. (A) Richness (observed OTUs). (B) Shannon’s diversity index. For freshwater reactor (FR): F3–F6 (FR_start) and F9–F14 (FR_end). For seawater reactor (SR): S3–S6 (SR_start), and S10–S13 (SR_end). Equal letters (a, b) above bars indicate no significant difference (p > 0.05) and different letters (a, b) indicate significant statistical differences (p < 0.05) by ANOVA and Tukey’s multiple comparisons. Error bars show standard error of the mean.