Table 2.
Results from SIMPER (similarity percentage) analysis showing the ten most significant OTUs contributing to the total difference between the four groups of samples in FR and SR reactors and the contribution of these OTUs to the difference between the four groups. Also included are the results from the classification of these OTUs based on QIIME pipeline and Greengenes, and phylogenetic analysis.
| OTU ID | SIMPER analysis results | Guild/(Classification (lowest taxonomic level) | Phylogenetic analysis (Maximum Composite likelihood method/Ribosomal Database Project) | Average relative abundance ± SD per sample of nitrifying reads (%) | ||||
|---|---|---|---|---|---|---|---|---|
| FR-samples | SR-samples | |||||||
| Contribution% | Cumulative% | Greengenes | FR-start (0‰) | FR-end (33‰) | SR-start (33‰) | SR-end (0‰) | ||
| 1124569 | 29.1 | 29.1 | NOB/Nitrospiraceae | Candidatus Nitrospira | 66.8 ± 8.7 | 24.0 ± 6.9 | 0.0 ± 0.0 | 6.3 ± 9.3 |
| 18274 | 23.8 | 52.9 | AOB/Nitrosomonadaceae | Nitrosococcus mobilis | 0.2 ± 0.2 | 12.2 ± 1.7 | 0.3 ± 0.5 | 77.8 ± 13.4 |
| 758679 | 15.4 | 68.3 | NOB/Nitrospiraceae | Candidatus Nitrospira salsa | 0.0 ± 0.0 | 0.2 ± 0.2 | 51.0 ± 18.5 | 0.9 ± 0.8 |
| 220928 | 6.5 | 74.9 | NOB/Bradyrhizobiaceae | Nitrobacter sp. | 13.8 ± 3.2 | 42.0 ± 4.7 | 0.1 ± 0.2 | 1.2 ± 0.9 |
| 6540 | 5.3 | 80.2 | AOB/Nitrosomonadaceae | Nitrosomonas sp. | 12.9 ± 8.0 | 7.2 ± 1.9 | 0.1 ± 0.0 | 0.0 ± 0.0 |
| 27445 | 4.4 | 84.7 | AOB/Nitrosomonadaceae | Nitrosomonas aestuarii | 0.0 ± 0.0 | 2.0 ± 3.2 | 14.8 ± 4.3 | 0.9 ± 0.5 |
| 345421 | 3.4 | 88.1 | NOB/Nitrospiraceae | Nitrospira marina | 0.0 ± 0.0 | 0.0 ± 0.0 | 10.9 ± 4.2 | 1.7 ± 1.0 |
| 1443 | 2.5 | 90.7 | NOB/Nitrospiraceae | Nitrospira marina | 0.0 ± 0.0 | 0.0 ± 0.0 | 8.3 ± 6.0 | 1.6 ± 2.3 |
| 265873 | 1.1 | 91.8 | AOB/Nitrosomonadaceae | Nitrosomonas cryotolerans | 0.0 ± 0.0 | 0.0 ± 0.0 | 2.8 ± 1.6 | 1.52 ± 0.6 |
| 2709955 | 0.9 | 92.7 | NOB/Nitrospiraceae | Nitrospira marina | 0.0 ± 0.0 | 0.0 ± 0.0 | 3.2 ± 0.9 | 0.0 ± 0.0 |