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. 2021 Feb 3;11:2923. doi: 10.1038/s41598-021-82617-2

Figure 5.

Figure 5

Effects of AdipoRon and leptin on the tube formation of MSS31 cells. (A) qRT-PCR analysis of the expression of AdipoRs and LepRs. (B) Effects of AdipoRon and leptin on the activation of AMPK, p38 MAPK and STAT3. The cells were incubated with 25 μg/ml AdipoRon or 0.5 μg/ml leptin for the indicated times. The uncropped images are shown in Supplementary Fig. S11-1. (C) Cell survival. The cells were incubated with various concentrations of AdipoRon for 2 days. Cell survival was measured with the MTT assay. The value for each point represents triplicate measurements. (D) Effects of AdipoRon and leptin on the tube formation of MSS31 cells. The cells were pretreated with leptin (100 ng/ml) for 1 h and then incubated with vehicle (DMSO) alone or 25 μg/ml AdipoRon plus 100 ng/ml leptin on Matrigel for 16 h. The number of branching points per field is also shown. Bars: 100 μm. *P < 0.01. (E) Effects of U0126 on AdipoRon-induced suppression of tube formation of MSS31 cells. The cells were pretreated with U0126 (10 μM) for 1 h and then incubated with vehicle (DMSO) alone or 25 μg/ml AdipoRon plus 10 μM U0126 on Matrigel for 16 h. The number of branching points per field is also shown. Bars: 100 μm. *P < 0.01. (F) Effects of leptin on AdipoRon-induced ERK1/2 activation in MSS31 cells. The cells were pretreated with leptin (100 ng/ml) for 1 h and then incubated with vehicle (DMSO) alone or 25 μg/ml AdipoRon plus 100 ng/ml leptin for the indicated times. The uncropped images are shown in Supplementary Fig. S11-2.