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. 2021 Feb 3;11:2974. doi: 10.1038/s41598-021-82685-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Peripheral and adipose tissue macrophage characteristics in uremia. (A) Peripheral blood flow cytometry showing the expression of different macrophage markers in adenine diet-induced mice versus control. (B) Adipose tissue flow cytometry showing the % of CD45+/CD64+ cells (macrophages) in adenine diet mice versus control mice. (C) Difference in % of expression of CD64+/CD11c+ and CD64+/CD11c- macrophage phenotypes in subcutaneous and visceral fat. (D) BUN level (mg/dL) in IL6 KO mice after adenine diet versus IL6 KO control mice. (E) Percentage of CD45+/CD64+ cells in adenine diet treated wild type and IL-6 KO mice; (F) Percentage of CD45+/CD64+ cells in IL-6 KO mice treated with adenine diet versus control. Unpaired t test, n = 4 for (B), (D), (E), n = 6 for (A), (C), error bars represent SEM *p ≤ 0.05. SF, subcutaneous fat. VF, visceral fat. Ade, adenine diet. Cont, control. KO, knockout.