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. 2021 Jan 21;8:622793. doi: 10.3389/fcell.2020.622793

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Copyright © 2021 Liu, Zhang, Kan, Wang, Liu, Gong and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of NKAP in neuroblastoma cell lines and verify of knockdown efficiency of NKAP. NB1 and SK-N-SH cells were transfected with siNKAP-1, 2, and 3. Non-specific lentiviral vectors with non-specific were used as negative controls (siNC). (A) mRNA levels of NKAP were detected by quantitative real-time PCR. (B) Protein levels of NKAP were detected by Western blot. (C) Quantitative real-time PCR assay was performed to test knockdown efficiency of NKAP. (D) Western blot assay was performed to test knockdown efficiency of NKAP. All data were expressed as mean ± standard deviation of three independent experiments. ***P < 0.001, **P < 0.01, *P < 0.05.