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. 2021 Jan 19;24(2):102069. doi: 10.1016/j.isci.2021.102069

Figure 1.

Figure 1

MS2-tagged glycolytic mRNAs are localized to granules in S. cerevisiae

(A) and (B) z-stacked images of strains expressing MS2-tagged mRNAs as labeled and the MS2 coat protein GFP fusion. Scale bar: 2 μm.

(C) Diagram of glucose fermentation to ethanol depicting the glycolytic mRNAs investigated in this study.

(D) A dotplot showing the variation in the number of granules per cell for each of the MS2-tagged strains above. n = 50. The mean ± SD are indicated for each strain.

(E) z-stacked images of smFISH performed on strains expressing MS2-tagged mRNAs and the MS2 coat protein GFP fusion. smFISH was performed for the canonical GPM1 gene (smGENE) or the MS2 stem loop sequence (smMS2). Scale bar: 3 μm.

(F) Beeswarm plot showing the proportion of smMS2 foci that colocalize with smGENE foci for a subset of strains expressing MS2-tagged glycolytic mRNAs. Each dot represents a single cell. n > 300.