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. 2021 Jan 19;24(2):102069. doi: 10.1016/j.isci.2021.102069

Figure 2.

Figure 2

smFISH analysis reveals that endogenous glycolytic mRNAs are present in multi-mRNA granules

(A) Upper diagram depicts the smFISH strategy. Multiple probes complementary to an mRNA (black) are tagged with a specific fluorophore (red). Lower panels show z-stacked smFISH images performed for the indicated endogenous glycolytic mRNAs. Both mRNAs (red) and nuclei (blue) are shown. Dotted lines represent the extent of the cell from brightfield micrographs. Scale bar: 3 μm.

(B) Beeswarm plot showing the number of multi-mRNA (>2.5mRNAs) granules per cell for a number of endogenous mRNAs. The gray box and line represent the interquartile range and the median, respectively. Each dot represents a single cell. n > 300.

(C) Beeswarm plot showing the proportion of mRNA that resides within multi-mRNA granules (>2.5mRNAs) per cell. Gray box and line represent the interquartile range and the median, respectively. n > 300.