Figure 4.

smFISH confirms that glycolytic mRNAs colocalize in granules

(A) z-stacked images from smFISH colocalization studies using the designated probes. Scale bar: 1 μm.

(B and C) (B) Diagram detailing the colocalization method used to generate data in panel. (C) The centroid of both spots must be within the radius of either channel in order for spots to be deemed colocalized (d < r₁|r₂). Spots that are touching are not always colocalized, if the distance between centroids is greater than the radius of both channels (d > r₁|r₂). (C) Beeswarm plot showing the proportion of colocalized smFISH foci considering total foci or only high-intensity foci, as indicated. Colocalization was assessed in a pairwise manner using smFISH foci identified via Fish-quant (see Methods). Simulated colocalization was assessed by sub-sampling foci properties across a number of pairwise comparisons (see Methods). Gray box and line represent the interquartile range and the median, respectively. Each data point represents a single cell, n > 300.