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. 2021 Jan 19;24(2):102069. doi: 10.1016/j.isci.2021.102069

Figure 7.

Figure 7

Human glycolytic mRNAs are present in granules and can colocalize

(A) Scatterplot of particles detected in z-stacked smFISH images of HeLa cells using probes to the mRNAs indicated. Images from three biological replicates were analyzed using the ImageJ ComDet plugin, which generates readouts of particle size (measured in pixels where each pixel = 45 × 45 nm) and the fluorescent intensity of particles.

(B) Single z-slice smFISH images of HeLa cells using probes to the mRNAs indicated. Scale bar: 10 μm. Insets magnified x2.

(C) Histogram showing the percentage of colocalized mRNA particles calculated using ComDet analysis of z-slices from three biological replicates. The significance across the various combinations was calculated using one-way ANOVA and the Tukey's HSD test. ENO2 versus PFKM (p value <0.05 shown by asterisk) is significantly different to ENO2 versus ENO1, ENO1 versus PFKM, or ENO2 versus LDHA. Error bars are ±SD.