Figure 4.

Hypercapnia impairs muscle regeneration, which is restored by etomoxir, in an in vivo cardiotoxin injury model. (A) Representative H&E images of tibialis anterior muscle from mice exposed to room air (RA) or high CO₂ for 21 days (H) injected at day 0 with PBS, 10 μl of 10 μM cardiotoxin (CTX) with or without pre-treatement with 25 mg/kg ETO. Bar = 20 μm. (B) Graphs shows fiber cross sectional area (CSA) from mice exposed and treated as in (A; n = 5–8 mice per condition). (C) Graphs shows the amount of centralized nuclei per number of fibers from mice exposed and treated as in (A; n = 5–8 mice per condition). Data from (B,C) were analyzed by one-way ANOVA followed by Tukey’s multiple comparison test. ^*p < 0.05; ^**p < 0.01; ^****p < 0.0001; n.s: not significant.