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. 2021 Jan 21;9:630645. doi: 10.3389/fcell.2021.630645

FIGURE 4.

FIGURE 4

Sema3E is promoted by MSCs-CM in retinal ganglion cells. (A) Sema3E levels were evaluated in lysates from RGC-5 cells subjected to MSCs-CM or vehicle for 48 h under hypoxic conditions. No changes were observed on RGC-5 cells treated with vehicle, whereas MSCs-CM treatment upregulated mRNA levels evaluated by qPCR (** = p < 0.01, * = p < 0.05 vs Vehicle, values are mean ± SEM, n = 4–5, pool of 2 retinas per n) and protein levels of Sema3E evaluated by Western blot (n = 4, pool of 2 retinas per n). (B) Representative photomicrographs from isolectin B4-stained retinal flatmounts from P17 OIR mice treated intravitreally with vehicle (PBS) or MSCs-CM in absence or presence of an anti-Sema3E antibody. Scale bar 500 μm. Histograms representing VO (C) and NV areas (D) that were significantly reduced in MSCs-CM respect to vehicle-treated, but prevented in the presence of the anti-Sema3E (**p < 0.01, ***p < 0.001 vs vehicle, values are mean ± SEM, n = 3–4 retinas).