Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 21;8:613621. doi: 10.3389/fbioe.2020.613621

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Hagen, Lehmann, Aurich, Bauer, Melzer, Moellerberndt, Patané, Schnabel and Burk.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

(A) Representative phase-contrast photomicrographs of equine mesenchymal stromal cells (MSC) from one donor at day 5 after seeding for population doubling assays in media supplemented with fetal bovine serum (FBS) or equine platelet lysate (ePL). (B–D) Confluent area at day 5, measured using Fiji ImageJ (B), generation times calculated from cell doubling numbers (C), and metabolic activity as determined by MTS tetrazolium-based cell proliferation assay (D) for MSC in passage 2 (P2) cultured in the different media; Friedman and Wilcoxon tests for group comparisons were performed (p-values are indicated); the plots display the individual values, median, and interquartile ranges. Data were obtained from MSC from n = 4 donors. Note that missing generation time data are due to the lack of proliferation in these samples (1 out of 4 in the 5% ePL group, 4 out of 4 in the 2.5% ePL group).