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. 2021 Jan 21;8:613621. doi: 10.3389/fbioe.2020.613621

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Copyright © 2021 Hagen, Lehmann, Aurich, Bauer, Melzer, Moellerberndt, Patané, Schnabel and Burk.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometry analysis of equine mesenchymal stromal cells (MSC) cultured with 10% fetal bovine serum (FBS) or 10% equine platelet lysate (ePL) after cells were mechanically detached and stained for 10 different surface markers. (A) Gating strategy for live MSC after duplet exclusion. (B) Representative examples of overlaid histograms of the surface marker staining and the respective isotype or conjugate control for MSC from one donor, and frequencies of surface marker positive MSC as % of live MSC plotted as individual values; horizontal bars mark the median and interquartile ranges. Data were obtained from MSC from n = 4 donors.