Table 3.
References | First centrifugation | Second centrifugation | Final PLT concentration | Lysis |
---|---|---|---|---|
Del Bue et al. (2007) | 350 × g, 10 min | 510 × g, 10 min | 1,000–2,000 × 109/L | Single freeze/thaw cycle + centrifugation (20,000 × g, 10 min) |
Seo et al. (2013) | 230 × g, 10 min, 10°C | 900 × g, 15 min, 10°C | 1,000 × 109/L | Single freeze/thaw cycle + centrifugation (1,600 × g, 30 min, 10°C) |
Russell and Koch (2016) | 200 × g, 15 min | 400 × g, 15 min | 1,000 × 109/L | Single freeze/thaw cycle + centrifugation (4,000 × g, 15 min) |
Yaneselli et al. (2019) | 200 × g, 10 min | 1,500 × g, 20 min | 591 × 109/L | Single freeze/thaw cycle + centrifugation (1,600 × g, 30 min) |
Bozorgmanesh et al. (2019) | 1,000 × g, 5 min 45 s | 2,000 × g, 8 min | 713,333 × 109/L | |
Gilbertie et al. (2018) | 250 × g, 15 min | 1,500 × g, 15 min | 1,226.38 × 109/L | 5 freeze/thaw cycles + centrifugation (20,000 × g, 20 min) |
Sumner et al. (2017) and Naskou et al. (2018) | Plateletpheresis | 357 ± 177 × 109/L | 2 freeze/thaw cycles + 3 centrifugation cycles (4,800 × g, 1.60 min and 2./3. 30 min) + centrifugation (3,485 × g, 10 min, 4°C) |