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. 2021 Jan 21;8:613621. doi: 10.3389/fbioe.2020.613621

Table 3.

Preparation technique for equine platelet (PLT) concentrate and PLT lysate and resulting PLT counts in the platelet concentrates in previous publications from 2007 to 2019.

References First centrifugation Second centrifugation Final PLT concentration Lysis
Del Bue et al. (2007) 350 × g, 10 min 510 × g, 10 min 1,000–2,000 × 109/L Single freeze/thaw cycle
+ centrifugation (20,000 × g, 10 min)
Seo et al. (2013) 230 × g, 10 min, 10°C 900 × g, 15 min, 10°C 1,000 × 109/L Single freeze/thaw cycle
+ centrifugation (1,600 × g, 30 min, 10°C)
Russell and Koch (2016) 200 × g, 15 min 400 × g, 15 min 1,000 × 109/L Single freeze/thaw cycle
+ centrifugation (4,000 × g, 15 min)
Yaneselli et al. (2019) 200 × g, 10 min 1,500 × g, 20 min 591 × 109/L Single freeze/thaw cycle
+ centrifugation (1,600 × g, 30 min)
Bozorgmanesh et al. (2019) 1,000 × g, 5 min 45 s 2,000 × g, 8 min 713,333 × 109/L
Gilbertie et al. (2018) 250 × g, 15 min 1,500 × g, 15 min 1,226.38 × 109/L 5 freeze/thaw cycles
+ centrifugation (20,000 × g, 20 min)
Sumner et al. (2017) and Naskou et al. (2018) Plateletpheresis 357 ± 177 × 109/L 2 freeze/thaw cycles + 3 centrifugation cycles (4,800 × g, 1.60 min and 2./3. 30 min)
+ centrifugation (3,485 × g, 10 min, 4°C)