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. 2021 Feb 3;11:2929. doi: 10.1038/s41598-021-82434-7

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of MDT and DST induced in vitro IFN-γ production. Blood samples from cattle experimentally infected with M. bovis (n = 22), cattle naturally infected with M. bovis (n = 21), naive controls (n = 59) and Gudair vaccinates (n = 29) were stimulated in vitro with PPDs, DST and MDT reagents and IFN-γ production measured by ELISA. Each symbol represents an individual animal while horizontal lines represent group medians. **p < 0.01, ***p < 0.001, ****p < 0.0001, Friedman test with Dunn’s multiple comparisons test.