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. 2021 Feb 3;11:2967. doi: 10.1038/s41598-021-82542-4

Figure 6.

Figure 6

Cytotoxic effects of NSC207895 are dependent on p53 signaling. (a) Immunoblotting with HepG2 and HepT1 cells transduced with sh-TP53 or control sh-Luc with an antibody recognizing p53. β-Actin immunoblotting was used as a loading control. Data shown are representative of at least three independent experiments. Full length blots for data shown in a are presented in Supplementary Fig. 10. (b) Cells transduced with sh-TP53 or control sh-Luc were incubated with varying indicated concentrations of NSC207895 for 48 h. MTT assays were performed at 48 h to assess viability. (c) Bar graphs representing normalized mRNA expression of p53 targets Bax, Puma, CDKN1A, and MDM2 analyzed with qPCR experiments. RNA was extracted from cells transduced with sh-TP53 or control sh-Luc treated with 10 μM NSC207895 for 0 or 4 h. Expression in treated cells (4 h) was compared to that from untreated cells (0 h). Data shown in b and c are representative of at least three independent experiments performed with three replicate wells each time. Error bars represent SD. Student’s t test (two tailed) *P < 0.05, **P < 0.01, ***P < 0.001.