Figure 7.

Cytotoxic effects of NSC207895 are dependent on MDM4. (a) Immunoblotting with HepG2 and HepT1 cells transduced with MDM4 cDNA or vector control (vc) with an antibody recognizing MDM4 (04–1555, Millipore). β-Actin immunoblotting was used as a loading control. Data shown are representative of at least three independent experiments. Full length blots for data shown in a are presented in Supplementary Fig. 11. (b) Bar graphs representing normalized mRNA expression of MDM4 analyzed with qPCR experiments. RNA extracted from HepG2 and HepT1 cells transduced with sh-MDM4-35 or sh-MDM4-37 was compared to that transduced with sh-GFP. (c) Cells transduced with MDM4 cDNA or vc were incubated with varying concentrations of NSC207895 for 48 h. MTT assays were performed at 48 h to asses viability. (d) Cells transduced with sh-MDM4 or control sh-GFP were grown for 120 h and MTT assays were done at 24 h intervals to assess cell number. Data shown in b, c, and d are representative of at least three independent experiments performed with three replicate wells each time. Error bars represent SD. Student’s t test *P < 0.05, **P < 0.01, ***P < 0.001.