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. 2021 Feb 3;11:2946. doi: 10.1038/s41598-021-82369-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

P. ankaliazontas and A. spiralis are prokinetoplastids. A maximum likelihood phylogeny of the 18S ribosomal RNA gene from diverse kinetoplastids and diplonemids (outgroup) was reconstructed with IQ-TREE v1.6.12 using the GTR + F + I + G4 model with 200 initial parsimony trees and 1000 ultrafast bootstrap replicates as a measure of support. P. ankaliazontas and A. spiralis affiliate with strong support with Prokinetoplastina, yet their precise relationships to parasitic and endosymbiotic prokinetoplastid groups could not be resolved. For clarity, ultrafast bootstrap values less than 85 are not shown. A phylogeny including more diverse euglenozoans as outgroup is presented in Supplementary Material, Fig. S1.