Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 3;12:781. doi: 10.1038/s41467-021-20980-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Representative images of longitudinal sections of the thoracic spinal cord show transduced cells after tail vein injection of different serotypes of AAVs expressing CAG-H2B-GFP at 3 h after T8 crush injury. Similar distributions were observed in all of 3 analysed mice for each AAV serotype. Scale bar: 1 mm. An asterisk represents lesion centre. b Quantification of GFP expression along the longitudinal axis. Mean ± SD are indicated by line and shadow with corresponding colours. a.u.: arbitrary unit. The fluorescence intensities were normalized to that in AAV2/9 (n = 3 mice for each AAV serotype, 5 sections per mouse). c Representative image showing the expression of H2B-GFP (cell bodies only) in different spinal levels. Similar distributions were observed in all of 3 analysed mice. Scale bar: 1 mm. d Magnification of the highlighted area one spinal segment away from the lesion (purple box in (c)), indicating co-localization of H2B-GFP and NeuN. Scale bar: 100 µm. e Representative image showing the expression of ChR2-tdTomato (cell bodies and axons) in different spinal levels. Similar distributions were observed in all of 3 analysed mice. Scale bar: 1 mm. f Cross section of the lumbar spinal cord showing descending ChR2-tdTomato+ axons from thoracic propriospinal neurons. Similar distributions were observed in all of 3 analysed mice. Scale bar: 500 µm.