TABLE 2.
Antiproliferative effects of RA esters and metabolites on VSMCs quantified by the resazurin conversion assay.
| Compound | VSMC proliferation at 10 μmol/L (relative to vehicle control, RU) | IC50 (µmol/L) |
|---|---|---|
| RA (6) | 0.781 ± 0.167n.s. | n.d. |
| RAME (7) | 0.415 ± 0.109 a | 7.90 |
| RAET (8) | 0.328 ± 0.081 a | 3.37 |
| RABU (9) | 0.308 ± 0.052 a | 2.84 |
| RAOCT (10) | 0.338 ± 0.147 a | 2.22 |
| RADOD (11) | 0.054 ± 0.081 a | n.d. |
| 1 (50 μmol/L) | 0.922 ± 0.124n.s. | – |
| 2 | 0.940 ± 0.220n.s. | – |
| 3 | 1.072 ± 0.152n.s. | – |
| 4 | 1.068 ± 0.173n.s. | – |
| 5 | 1.205 ± 0.278n.s. | – |
Results are presented as mean ± SD, relative to the PDGF-BB-stimulated vehicle control, and IC50 values were determined if the compound (10 μmol/L) showed a relative inhibitory action lower than 0.75 RU. All values were obtained from a minimum of three independent experiments.
a
p < 0.001; n.s. not significant compared to vehicle control.
–not tested; n.d.: unable to calculate.