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In vitro biomimetic trachea regeneration. Gross view of the biomimetic trachea after 4 weeks in vitro culture (A,B). (C) shows the sectioning schematic. HE, safranin-O, collagen II, and toluidine blue staining of a longitudinal section of the engineered biomimetic trachea (D1–G1). Magnified views of a cartilage ring (D2–G2) and PCL ring (D3–G3). The green arrows indicate cartilage regions, and the yellow arrows indicate PCL regions.
