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In vivo biomimetic trachea formation and histological analysis of a longitudinal section. Gross view of the biomimetic trachea after 6 weeks in vivo incubation (A,B). (C) shows the sectioning schematic. HE, safranin-O, collagen II, and toluidine blue staining of a longitudinal section of the engineered biomimetic trachea (D1–G1). Magnified views of the cartilage ring (D2–G2) and PCL ring (D3–G3). The green arrows indicate cartilage regions, and the yellow arrows indicate PCL regions.
