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. 2021 Jan 21;9:626027. doi: 10.3389/fcell.2021.626027

FIGURE 5.

FIGURE 5

circCOL5A1 regulated HKFs proliferation, migration, apoptosis, and ECM deposition through circCOL5A1/miR-7-5p/Epac1 axis. (A) CCK-8 assays and (B) EdU assays were performed to evaluate the proliferation ability in HKFs transfected with the si-NC, si-circ, or si-circ + inhibitor, respectively. Magnification, 200×. (C) Transwell migration assays (magnification, 200×) and (D,E) wound healing assays (magnification, 20×) were applied for assessing the migration ability of HKFs transfected with the si-NC, si-circ, or si-circ + inhibitor, respectively. (F) Cell apoptosis was examined using flow cytometry. (G,H) The protein levels of collagen I, collagen III, α-SMA, and the protein phosphorylation levels of Akt and PI3K in HKFs transfected with si-NC, si-circ, or si-circ + inhibitor by western blot assays. Data was shown as mean ± SD. ns indicated no significance, #P < 0.05, ##P < 0.01, ###P < 0.001, vs. si-NC.