Table 1.

Primer sequences and PCR conditions used for the detection of aflatoxigenic Aspergillus isolated from dairy feeds.

Prime pair	Target gene	Primer sequence (5' → 3')	PCR conditions							Reference
			1	2	3	4	5	6	Product size (bp)
Nor-1F Nor-1R	aflD	ACCGCTACGCCGGCACTCTCGGCAC GTTGGCCGCCAGCTTCGACACTCCG	94°C:10 min	94°C:1 min	65°C:1 min	72°C:2 min	33	72°C:min s	400
Ver-1F Ver-1R	aflM	GCCGCAGGCCGCGGAGAAAGTGGT GGGGATATACTCCCGCGACACAGCC	95°C:4 min	95°C:1 min	58°C:1 min	72°C:30s	30	72°C:10 min	600	Rashid et al., 2008; Abd El-Aziz et al., 2015
Omt-1F Omt-1R	aflP	GTGGACGGACCTAGTCCGACATCAC GTCGGCGCCACGCACTGGGTTGGGG	94°C:5 min	94°C:1 min	75°C:2 min	72°C:2 min	33	72°C:10 min	797
aflR-1F	aflR	TATCTCCCCCCGGGCATCTCCCGG GTCGGCGCCACGCACTGGGTTGGGG	95°C:4 min	94°C:1 min	60°C:1 min	72°C:30s	30	72°C:10 min	1,000
aflR-1R
AflS-1F AflS-2R	aflS	TGAATCCGTACCCTTTGAGG GGAATGGGATGGAGATGAGA	95°C:10 min	95°C:50s	58°C:50s	72°C:2 min	30	72°C:5 min	684	Gallo et al., 2012; Fakruddin et al., 2015

1, Pre-dwelling; 2, Denaturation; 3, Annealing; 4, Extension; 5, Cycles; 6, Post-dwelling.