Table 2.
Primers used in this study.
| Primers | Sequences (5’-3’) | References |
|---|---|---|
| Construction of mutant and complementary strain | ||
| qseC-LF | TCGAAGCTTAGAAGCGAAAGTGTATTTGAC | This study |
| qseC-LR | CTATTGTTGCGCGTTTAATGGAGTAAGGTTT | |
| qseC-RF | CATTAAACGCGCAACAATAGAGCTGATTCC | This study |
| qseC-RR | CATAAGCTTTTTCGGGTAGATGAGGTATTC | |
| Check-F | GCAGAAGAAGTCATTCAAGTT | This study |
| Check-R | GGATGAACAACCAGCATAAC | |
| C-F | CATCTTAAGGAACTATTTTTATGGAGACTTCCTTG | This study |
| C-R | CTTCTCGAGCTAGTACTTTGGGAATGCGAC | |
| Quantitative real-time reverse transcription PCR | ||
| recA-F | GCTAGTAGAAAAAGCGGGTG | Ma et al., 2015 |
| recA-R | GCAGGTGCTTCTGGTTGAG | |
| qseC-F | TCAGCCAGAACAGCAACA | This study |
| qseC-R | AGACCGAGCATGAGAACG | |
| flaC-F | ACCGTATCGCAGAAACCACA | This study |
| flaC-R | GCCAGGAAGCGTGATTTTTA | |
| flaF-F | TTTGGATGTGGCGGTTCG | This study |
| flaF-R | GCTCGGCTTGGCTGTTTG | |
| flaK-F | GCGTATGGTCAGGCTGTATC | This study |
| flaK-R | TGTGGGTAATTTAGCGGTTG | |
| fliA-F | CCGACGAGCAAGATTATTACA | This study |
| fliA-R | ATCCAAGACGAGGGCTATTT | |
| motB-F | CTTTACGGTGCGGTGTTG | This study |
| motB-R | GCGGTTAAGCGTTTCTTG | |
| cpsA-F | GTATTACTCCGTTTGGTGG | This study |
| cpsA-R | CTGGTTTGACTTTGTGGC | |
| vasD-F | ATGCTCCGCCGCTAAT | This study |
| vasD-R | ACAACTGGTGATGGTCTTC | |