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. 2021 Jan 21;11:591398. doi: 10.3389/fphar.2020.591398

FIGURE 1.

FIGURE 1

SB290157 potently activates C3aR-mediated ERK signaling in transfected CHO cells. (A) Agonism testing of SB290157 on CHO-C3aR cells. Serum-starved CHO-C3aR cells (50,000/well) were stimulated with respective concentrations of SB290157 or purified human C3a for 10 min before being lysed. (B) Agonism testing of SB290157 on CHO-C5aR1 cells. Serum-starved CHO-C5aR1 cells (50,000/well) were stimulated with the respective ligands at the indicated concentrations for 10 min before being lysed. (C) Antagonism testing of SB290157 on CHO-C5aR1 cells. Serum-starved CHO-C5aR1 cells (50,000/well) were pre-treated with SB290157 (10 µM) or vehicle (0.1% DMSO) for 30 min before being stimulated with 0.3 nM of C5a for 10 min and then lysed. The phospho-ERK1/2 content in the cell lysate was measured and normalised to the maximum C3a-induced (for A) or 0.3 nM C5a-induced (for B, C) levels before being combined. Data represent mean ± S.E.M. of triplicate measurements from 3 to 6 independent experiments (n = 3–6).