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. 2021 Jan 21;11:512581. doi: 10.3389/fmicb.2020.512581

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Copyright © 2021 Yang, Yang, Chen, Li, Zhang, Chen, Zhang, Mai and Zeng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Commensal bacteria regulate miRNA expression in cecum and lungs. Total RNA, including low molecular weight RNA, was prepared from the cecum and lungs derived from mice with or without antibiotics treatment. The expression of miRNA was analyzed by t-test. Volcano Plots compare the expression of miRNAs between cecum in mice with or without antibiotics treatment (A) or between lungs in mice with or without antibiotics treatment (B). The most significantly differentially expressed miRNAs with a greater than 2-fold difference in expression in cecum and lungs are shown in panels (C,D).