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. 2021 Jan 21;11:512581. doi: 10.3389/fmicb.2020.512581

FIGURE 3.

FIGURE 3

MiR-21-3p expression deregulated by bacterial components is induced by M. tuberculosis infection in humans and mice. (A) MiR-21-3p expression in PBMCs from healthy controls (N = 21) and active TB patients (N = 31) was analyzed by qPCR. (B) Splenocytes from mice were stimulated with Medium (N = 7) or M. tuberculosis antigen (N = 6) for 72 h. MiR-21-3p expression was analyzed by qPCR. Normalized values of miR-21-3p with or without stimulation are shown as the mean ± SD of 3 independent experiments. (C) MiR-21-3p expression of representative lungs derived from the M. tuberculosis-infected mice with treatment of water (N = 5), antibiotics (N = 5) or FMTs of gut microbiota from healthy individuals (N = 5), respectively. Data shown as mean ± SEM are representative of two independent experiments. For human study, N > 20/per group, **p < 0.01, Student t-test. For animal experiments, N = 5-7 mice/per group *p < 0.05, non-parametric Mann–Whitney U-test.