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Binding affinity of ligand 3 to E protein. (A) STD build up curves at different saturation time to determine STDtotal using Eqs. (4), (5). (B) Dissociation constant (KD) were calculated for E protein/ligand 3 complex using hill equation (Eq. (6)) using prism 9. (C) Binding of ligand 3 to C terminal E protein NR25 (N45-R69) at a molar ratio of NR25:ligand 3 = 1:100. The experiment was performed at 298 K using Bruker Avance III 700 MHz.
