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. 2021 Jan;9(1):42. doi: 10.21037/atm-20-1013

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Identification of CMTM8 as an LPA1-interacting protein. (A) PANC-1 and BxPC-3 cells were exposed to 10 μM lysophosphatidic acid (LPA) and immunoprecipitated with anti-LPA1 antibody or isotope control IgG and probed for indicated proteins by Western blotting. (B) Detection of LPA1 protein by Western blotting in CMTM8 immunoprecipitates from BxPC-3 and PANC-1 cells. (C) Confocal microscopy indicated that LPA1 and CMTM8 were co-localized in pancreatic cancer cells. Scale bar =10 μm. (D) Western blot analysis of LPA1 and CMTM8 in pancreatic cancer cell lines. All experiments were performed three times.