Table 1. Metastats and LEfSe analyses of differential microbes at different levels of biological classification.
| Bacteria | Metastats analysis | LEfSe analysis | ||||
|---|---|---|---|---|---|---|
| HC (mean) | IgAN (mean) | t-test | HC (LDA value) | IgAN (LDA value) | ||
| Phylum | ||||||
| Firmicutes | 60.1% | 64.9% | 0.210 | <2 | ||
| Bacteroidetes | 23.20% | 9.81% | <0.01 | 4.98 | – | |
| Proteobacteria | 7.97% | 15.00% | 0.045 | – | <2 | |
| Class | ||||||
| Clostridia | 51.8% | 47.4% | 0.425 | <2 | ||
| Bacteroidia | 23.20% | 9.81% | 0.001 | 4.98 | – | |
| Bacilli | 1.4% | 11.6% | 0.054 | 4.65 | ||
| Order | ||||||
| Clostridiales | 51.8% | 47.4% | 0.425 | <2 | ||
| Bacteroidiales | 23.20% | 9.81% | 0.001 | 4.98 | – | |
| Lactobacillales | 1.39% | 11.6% | 0.052 | 4.64 | ||
| Family | ||||||
| Bacteroidaceae | 11.30% | 4.48% | 0.018 | 4.532 | – | |
| Enterococcaceae | 0.03% | 5.08% | 0.009 | – | 4.48 | |
| Streptococcaceae | 0.78% | 5.76% | 0.021 | – | 4.415 | |
| Genus | ||||||
| Faecalibacterium | 15.3% | 9.5% | 0.048 | <2 | ||
| Streptococcus | 0.77% | 8.6% | <0.01 | – | 4.415 | |
| Blautia | 4.56% | 10.20% | 0.030 | – | <2 | |
| Bacteroides | 11.30% | 4.30% | 0.020 | 4.61 | – | |
| Enterococcus | 0.10% | 6.70% | <0.001 | – | 4.48 | |
HC, healthy control; IgAN, immunoglobulin A nephropathy; LDA, linear discriminant analysis; LEfSe, linear discriminant analysis of effect size; Note: Microbes with LDA values >2 and t-test P values <0.05 were considered statistically significantly different.