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. 2021 Jan;9(1):18. doi: 10.21037/atm-20-3810

Figure 4.

Figure 4

Treatment of sEphB4 inhibits VEGFR2 (vascular endothelial growth factor receptor 2) phosphorylation in rat retina. The expression of VEGFR2 (A) and pVEGFR2 (phosphorylated vascular endothelial growth factor receptor 2) (B) in laser induced CNV lesion after laser days 14. VEGFR2 stained as green and nuclear counterstain as red. There is no significant difference in the expression of total VEGFR2 between PBS (phosphate-buffered saline) and sEphB4-HSA injection (A); however, the pVEGFR2 expression was much weaker (B) in sEphB4-HSA injected rats compared with controls. Scale bar: 25 µm. (C) Western blot analysis showed phosphorylation of VEGFR2 in the eye with the sEphB4 injection was significantly reduced compared with PBS group, however, total VEGFR2 protein levels remained the same. (D) Quantitative analyses of the data were representative of three independent experiments. Values represent mean ± SD, and the P value was shown in the figure.