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. 2020 Dec 17;62:100013. doi: 10.1194/jlr.RA120000737

Fig. 1.

Fig. 1

Generation of Lpcat3KOMac mice and lipidomic characterization of Lpcat3-deficient macrophages. A: Lpcat3 targeting vector. A gene-trap LacZ cassette is located downstream of exon 2 of the Lpcat3 gene. B: Relative Lpcat3 mRNA levels in macrophages derived from WT and Lpcat3KOMac mice (four independent mice in each group). Data are expressed as mean ± SEM (∗P < 0.05 vs. WT Mann-Whitney test). C: Changes of lipidomic profile of Lpcat3KOMac versus WT macrophages (four independent mice in each group, P value at 0.01 and ±0.6 log-fold changes as cut off). D–J: Fatty acid composition of phosphatidylcholines (D), phosphatidylethanolamines (E), plasmalogens (F), phosphatidylserines (G), phosphatidylinositols (H), cholesterol esters (I), and free fatty acids (J) of WT and Lpcat3KOMac macrophages (n = 4 in each group). Data are expressed as mean + SEM (∗P < 0.05 vs. WT Mann-Whitney test).