Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 11;45(3):945–962. doi: 10.3892/or.2021.7930

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Szczygieł et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 2. — Modulation phenotype of mature DCs generated in the presence of metabolites released by MC38 cells after MTX or HES-MTX treatment. (A) Percentage of Annexin V⁺ MC38 cells after a 72-h treatment with MTX or HES-MTX as determined by Annexin V binding assay; (B) Bar plots showing the percentage of CD11c⁺ mature DCs (stimulated with TAg) (C) Bar plots and representative histograms showing the expression of MHC II and costimulatory molecules on mature DCs. Results are expressed as mean ± SD calculated for three independent experiments. Differences between groups were calculated using (A) the Brown-Forsythe and Welch ANOVA test followed by Dunnett's T3 multiple comparisons post-hoc test or (B and C) one-way ANOVA followed by Tukey's multiple comparison post-hoc test. The asterisks (*) presented in the graphs indicate statistically significant differences between the given groups; a hashtag (^#) above a bar indicates a statistically significant difference between the given group and the control group-DC/TAg (^*/#P<0.05, ^**/##P<0.01, ^***/###P<0.001 and ^****/####P<0.0001). HES, hydroxyethyl starch; MTX, methotrexate; DCs, dendritic cells; TAg, tumor antigen.