Table 2. Temperature-Dependent Binding Data Obtained from ITC Experimentsa.
| temperature (K) | K × 105 (M–1) | ΔH (cal/mol) | ΔCp (cal/mol K) | ΔGhyd (cal/mol) | ΔSASA (Å)2 |
|---|---|---|---|---|---|
| 288 | 7.06 | –615 | –160.5 ± 3.17 | –12840 ± 253.6 | 584 ± 11.52 |
| 293 | 5.50 | –2165 | |||
| 308 | 3.50 | –3825 |
a
All the data in this table are derived from the ITC studies and are average of four determinations. T, K, and ΔH denote absolute temperature, binding constant, and enthalpy change, respectively. Gibbs free energy change (ΔG) was determined using the equations ΔG = −RT ln K.