TABLE 2.

Primer sequences for quantitative real-time polymerase chain reaction.

Gene	Forward primer (5′-3′)	Reverse primer (5′-3′)
β-actin (mouse and rat)	ACT​CTT​CCA​GCC​TTC​CTT​C	ATC​TCC​TTC​TGC​ATC​CTG​TC
GnRH (mouse)	GGG​AAG​ACA​TCA​GTG​TCC​CAG	CTC​GAG​CTT​CCG​TTG​GTA​GG
GPR54 (mouse)	CTG​TCA​GCC​TCA​GCA​TCT​GG	AGC​AGC​GGC​AGC​AGA​TAT​AG
Erα (mouse)	AAG​ACG​CTC​TTG​AAC​CAG​CA	CGA​GTT​ACA​GAC​TGG​CTC​CC
Erβ (mouse)	AAG​ACG​CTC​TTG​AAC​CAG​CA	CGA​GTT​ACA​GAC​TGG​CTC​CC
GnRH (rat)	TGG​TAT​CCC​TTT​GGC​TTT​CAC​A	TGA​TCC​TCC​TCC​TTG​CCC​AT
Kiss1 (rat)	CAA​TGG​TCT​GAA​CTG​CCC​AC	CAC​AGG​TGC​CAT​TTT​TGC​CA
GPR54 (rat)	CAA​CCT​GCT​GGC​CCT​ATA​CC	CTAGCAGCTGCAGGGCG

GnRH, gonadotropin-releasing hormone; Kiss1, Kiss-1 metastasis suppressor; GPR54, G-protein coupled receptor 54; Erα, estrogen receptor alpha; Erβ, estrogen receptor beta.