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Decreased Rubicon protein expression in ASHE-treated liver cancer cells. Protein expression of (A) Rubicon and (B) Rubicon-related proteins. HuH-7 and HepG2 cells were treated with ASHE at the indicated concentrations in absence of FBS. Protein expression was detected by western blotting. Actin was used as the loading control. ASHE, Acanthopanax senticosus Harms root extract; p-, phospho-; Rubicon, run domain Beclin-1-interacting and cysteine-rich domain-containing.
