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. 2021 Jan 26;13(1):115–127. doi: 10.4252/wjsc.v13.i1.115

Table 1.

Primer sequences utilized for real-time polymerase chain reaction

Gene
5’-3’
Primer sequence
Product size (bp)
Reference sequence
Col1a1 Forward Reverse GGAGAGAGTGCCAACTCCAG; GTGCTTTGGAAAATGGTGCT 182 NM_053304.1
Col3a1 Forward Reverse TCCCAGAACATTACATACCACT; GCTATTTCCTTCAGCCTTGA 126 NM_032085.1
Tnc Forward Reverse AGATGCTACTCCAGACGGTTTC; CACGGCTTATTCCATAGAGTTCA 200 NM_053861.1
Scx Forward Reverse AACACGGCCTTCACTGCGCTG; CAGTAGCACGTTGCCCAGGTG 123 NM_001130508.1
Tnmd Forward Reverse GTGGTCCCACAAGTGAAGGT; GTCTTCCTCGCTTGCTTGTC 60 NM_022290.1
PPARγ Forward Reverse CAGGCTTGCTGAACGTGAAG; ACGTGCTCTGTGACAATCTGC 157 NM_001145366.1
Runx2 Forward Reverse CAGACCAGCAGCACTCCATA; CAGCGTCAACACCATCATTC 178 NM_001278483.1
GAPDH Forward Reverse AAGTTCAACGGCACAGTCAAGG; CGCCAGTAGACTCCACGACATA 139 NM_017008.4